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Key regulator of mitochondrial calcium uptake required for calcium entry into mitochondrion
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACTN2
The OD value is proportional to the concentration of Rat RBP4
Then a biotinylated detection antibody specific for Mouse TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated F9 antibody
Human CFL1 (Cofilin-1) ELISA Kit ABP1 Key regulator of mitochondrial calciumThe Human (CFL1) Cofilin 1 ELISA Kit measures Cofilin 1 in samples. The plate has been pre coated with Human CFL1 antibody. CFL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CFL1 Antibody is added and binds to CFL1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CFL1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
