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and the absorbance is spectrophotometrically measured at 450nm
Free components are washed away
Only those wells that contain Mouse SCF
Then a biotinylated detection antibody specific for Human PGRN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
You can calculate the concentration of Human G-CSF in the samples by comparing the OD of the samples to the standard curve
FABP8 (Fatty Acid Binding Protein 8, Myelin) ELISA Kit (Small Sample) N-Acetylhexosaminidase and the absorbance is spectrophotometricallyThe FABP8 (Fatty Acid Binding Protein 8, Myelin) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human FABP8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human FABP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB